1 microscopy and cytology abstract in todays lab you will be viewing tissue samples from plants and animals under the microscope and identifying the various cellular organelles found in each cell type. Laboratory report 2 basic medical microbiology sbp3403 staining of bacteria, microscopy and microscopic measurement lecturer: prof dr mohd nasir mohd desa date : 26 september 2013 cell biology unit department of biomedical and health sciences faculty of medicine and health sciences universiti putra malaysia learning objectives 1. Gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884 gram staining is still the cornerstone of bacterial identification and taxonomic division. The last staining technique that we used are the gram stain technique this technique are different than the other two because this technique splits the bacteria into two groups, gram- positive and gram-negative. Learn simple stain microbiology lab with free interactive flashcards choose from 500 different sets of simple stain microbiology lab flashcards on quizlet.
The simple stain can be used as a quick and easy way to determine cell shape, size and arrangements of bacteria true to its name, the simple stain is a very simple staining procedure involving single solution of stain. This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. Introduction to microscopy conclusion the light microscope is a very powerful tool for understanding the structure and function of tissues, and it is widely used in biomedical science courses, as well as in research and diagnostic laboratories.
Lab 1 microscope and simple stain - free download as powerpoint presentation (ppt), pdf file (pdf), text file (txt) or view presentation slides online scribd is the world's largest social reading and publishing site. Cellular and molecular techniques - lab #1 bio 349 lab report #1 microscopy and staining abstract the primary focus of this lab was on microscopy and simple stains. Learn micro lab quiz gram stain smears simple with free interactive flashcards choose from 500 different sets of micro lab quiz gram stain smears simple flashcards on quizlet.
Microscope is to be handled very carefully, it should be lifted with two hands and the electric cord of the microscope should be coiled before keeping it to the safe place so this lab was done to introduce the basic concepts about different types of electron microscopes and to make their use for viewing different objects. Microscopy lab report 1 introduction microscopy is an essential technique not only in cell biology but natural science as a whole we compared different types of microscopic techniques , according to the specimen used and the scope of the experiment. Exercise 3 preparation of smears and simple staining 1 which bacterium is a rod answer: bacillus megaterium 2 of what value is a simple stain answer: the value of simple stain is to allow us the determination of cell morphology, size and arrangement of organisms with the application of only one reagent such as methylene. Staining provides a contrast between different structures in a tissue specimen and allows its examination under a light microscope (cook, 2006) haematoxylin and eosin (h&e) is a routine stain that is used to microscopically diagnose a vast majority of specimens in which the haematoxylin stains the nuclei, whereas the eosin is used to stain. Fe204 microbiology lab report the gram stain and simple stain smear purpose preparation of smear and application of simple stain to observe size,shape, arrangement, color of the microorganisms using oil immersion objectiveand application of differential staining method and classify microorganism as gram positive, gram negative.
The purpose of this lab is to learn how to prepare a wet mound, to learn proper staining techniques and to examine human cheek cells and onion skin cells it is predicted that all cells, no matter if it be plant or animal, will be in beside each other in large groups, that most of the major. Gram staining is a differential staining technique that differentiates bacteria into two groups: gram-positives and gram-negatives the procedure is based on the ability of microorganisms to retain color of the stains used during the gram stain reaction. 3 laboratory report sheet questions: 1 record your results of staining e coli staphylococcus 2 since everything on the slide will be the same color in simple staining, what you. For more information, visit this video demonstrates the gram staining method for identifying unknown bacter. Staining and 3) will retain the original cell morphology after fixation and staining after you have stained your bacterial smears, you will examine them with the oil immersion lens, noting the morphological and staining characteristics of each species.
27 lab 3 results i differential stains a gram stain draw and label examples of escherichia coli and staphylococcus epidermidis b acid-fast stain draw and label examples of mycobacterium smegmatis and staphylococcus epidermidis. Bio 3a lab scopes & cells page 3 of 13 j on/off knob/switch: located on side of the microscope (nikon) or the top. Microscopy refers to the practice that involves the use of a microscope for the purposes of observing small scale structures that cannot be viewed using the naked eye and often cell staining is necessary as s tructures are difficult to discern due to insufficient contrast.
Lab 2: staining and streaking protocols for simple stain, gram stain, streak plate technique and culture maintenance lab 2a: introduction to staining live specimens are difficult to see with the bright field microscope. Methods could also be referred to the lab manual pages 159-163 microscope e1 was used to view unknown number 20 to determine whether the bacteria were gram-positive or gram-negative and cocci or bacillus.